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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: Methods Cell Biol. 2021 Jul 30;167:171–183. doi: 10.1016/bs.mcb.2021.06.014

Fig 2. Gammaretroviral gene transfer overview:

Fig 2.

(A) Outline of the production of gammaretroviral particles. Transfection of transfer plasmid and packaging plasmids into 293T cells results in the production of gammaretroviral particles in the culture superatant. (B) Outline of CAR T cell generation process. PBMCs are stimulated with CD3/CD28 to promote their proliferation (topline). Culture supernatant containing gammaretroviral particles is added to Retronectin-coated plate and then centrifuged (bottom line). Activated T cells are added onto gammaretroviral particle-bound Retronectin plate for transduction. *Timeline can be modified.