(A) TLC quantification of hair lipids from Wt versus Tslpr−/− mice at baseline (n=11 mice/group, pooled from 3 independent experiments). t-test. (B) Ki67 quantification of sebaceous glands from Wt versus Tslpr−/− mice (n=132 Wt and 109 Tslpr−/− sebaceous glands from three mice/group, pooled from 2 independent experiments). t-test. (C) TLC quantification of hair lipids from Wt versus Ebeta−/− mice at baseline (n=7–8 mice/group, pooled from 3 independent experiments). t-test. (D) Expression of sebum-associated AMPs in the skin (n=16 mice/group, qPCR normalized to Hprt expression, pooled from 3 independent experiments). t-test. (E) Correlation analysis of sebaceous gland (SG) gene expression versus TSLP expression in human skin (n=36 healthy subjects). Pearson correlation and linear regression slope test. (F) Model for pharmacologic and homeostatic roles of TSLP-driven sebum secretion. N.S., P≥0.05, *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. Data are mean ± SEM.