Table 2.

Primer sequences for real-time qPCR

Gene	Primer Sequences (5′ to 3′)
Markers of differentiation
FOXJ1	hqFoxJ1-F1: ATCTACAAGTGGATCACGGAC hqFoxJ1-R1: GAGGCACTTTGATGAAGCAC
MCIDAS	hqMcidas-F1: TGAGCAATACTGGAAGGAGG hqMcidas-R1: CCTGTTTCTGGGTCAATGTC
TP63	hqP63-F1: CGTGAGACTTATGAAATGCTG hqP63-R1: TGAAGATGGAGACTGTATTGAG
KRT5	hqKrt5-F1: GCAGTACATCAACAACCTCAG hqKrt5-R1: CTACATCCTTCTTCAGCATCAC
SCGB1A1	hqCCSP-F1: CCTGATCAAGACATGAGGGA hqCCSP-R1: TAATTACACAGTGAGCTTTGGG
CGRP	hqCGRP-F1: GGACTATGTGCAGATGAAGG hqCGRP-R1: CGTGTGAAACTTGTTGAAGTC
Secretory/goblet cells
MUC5AC	hqMuc5AC-F1: GGAACTTCAACAGCATCCAG hqMuc5AC-R1: GAGCATACTTCTCATTCTCCAC
MUC5B	hqMUC5B-F1: TGTTCCTCAACTCCATCTACAC hqMUC5B-R1: CTGACAAACACCTGCATGAG
MUC1	hqMUC1-F1: AACCTCCAGTTTAATTCCTCTC hqMUC1-R1: AATTGTACCACCACAGATCC
Ion channels
TMEM16	hqTMEM16-F1: AGACTCTCCTATCCCTTCTCC hqTMEM16-R1: TCTCATAGACAATCGTGCTCC
SCNN1A	hqENaC-F1: TCTCTGCTGGTTACTCACGA hqENaC-R1: GCTCCTTGAAGAAGATGTTGAC
ATP1A1	hqNaKATPase-F1: ATGTCTATGATTGACCCTCCC hqNaKATPase-R1: CCCTCTGATATGATGCCCAC
CFTR	hqCFTR-F1: CCTATGACCCGGATAACAAGGA hqCFTR-R1: GAACACGGCTTGACAGCTTTA
ATP12A	hqATP12a-F1: GGCACAGACATCATTATGGGTC hqATP12a-R1: GATGCAGACTTGTCGCTGG
Reference gene
GAPDH	hqGAPDH-F1: GGAGCGAGATCCCTCCAAAAT (Tm 61.6) hqGAPDH-R1: GGCTGTTGTCATACTTCTCATGG (Tm 60.9)

FOXJ1, Forkhead box J1; MCIDAS, multiciliate differentiation and DNA synthesis associated cell cycle protein; TP63, tumor protein P63; KRT5, keratin 5; SCGB1A1, secretoglobin family 1A member 1; CGRP, calcitonin gene-related peptide; MUC5AC, Mucin 5AC; MUC5B, Mucin 5B; MUC1, Mucin 1; TMEM16, transmembrane protein 16; SCNN1A, sodium channel epithelial 1 subunit alpha; ATP1A1, ATPase Na⁺/K⁺ transporting subunit alpha 1; CFTR, cystic fibrosis transmembrane conductance regulator; ATP12A, ATPase H⁺/K⁺ transporting, nongastric, alpha polypeptide; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.