Table 3.
Clinical validation of CRISPR-SPADE with non-N gene for detecting SARS-CoV-2.
| Non-N gene for detecting SARS-CoV-2 VOC |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Genomic sequencing results |
Total | |||||||||
| α | β | γ | δ | ο | Other | |||||
| SPADE | α | Positive | 31 (TP) | 0 (FP) | 0 (FP) | 0 (FP) | ·· | 0 (FP) | 31 | |
| Negative | 2 (FN) | 0 (TN) | 2 (TN) | 11 (TN) | ·· | 16 (TN) | 31 | |||
| β | Positive | 0 (FP) | 1 (TP) | 0 (FP) | 0 (FP) | ·· | 0 (FP) | 1 | ||
| Negative | 15 (TN) | 0 (FN) | 2 (TN) | 11 (TN) | ·· | 16 (TN) | 44 | |||
| γ | Positive | 0 (FP) | 0 (FP) | 13 (TP) | 0 (FP) | ·· | 0 (FP) | 13 | ||
| Negative | 15 (TN) | 0 (TN) | 4 (FN) | 11 (TN) | ·· | 16 (TN) | 46 | |||
| δ | Positive | 1 (FP) | 0 (FP) | 0 (FP) | 53 (TP) | ·· | 0 (FP) | 54 | ||
| Negative | 15 (TN) | 0 (TN) | 2 (TN) | 3 (FN) | ·· | 16 (TN) | 36 | |||
| ο | Positive | ·· | ·· | ·· | ·· | ·· | 2 | |||
| Negative | ·· | ·· | ·· | ·· | ·· | ·· | ·· | |||
| Other | Positive | 0 (FP) | 0 (FP) | 0 (FP) | 0 (FP) | ·· | 16 (TP) | 16 | ||
| Negative | 15 (TN) | 0 (TN) | 2 (TN) | 11 (TN) | ·· | 0 (FN) | 28 | |||
| Total | 94 | 1 | 25 | 100 | 2 | 80 | 302 | |||
TP = true positive; TN = true negative; FP = false positive; FN = false negative