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Conjugation of RBD with maleimide-containing liposomes. The conjugation of purified RBD proteins with maleimide on the surface of liposomes was monitored using a 15% (29:1 acrylamide/Bis) reducing SDS-polyacrylamide gel followed by silver staining. Lane 1: molecular weight marker. Lane 2: the RBD–liposome conjugate before SEC. Lane 3: the admixture of RBD-Ala and liposome under the same conditions as RBD–liposome in lane 2. Lane 4: SEC-purified RBD–liposome.
