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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: Biomaterials. 2021 Dec 11;280:121318. doi: 10.1016/j.biomaterials.2021.121318

Figure 6: O2 delivery from μtank scaffolds increases matrix and osteogenic protein deposition.

Figure 6:

Unloaded or O2-loaded 20% μtank scaffolds and ASCs were implanted into 4 mm murine calvarial defects for 8 weeks. (A, B) μCT of calvarial defects with scaffolds pseudocolored magenta and compared to empty defects in the contralateral side; scale bar = 4 mm. (C) Quantification of bone volume normalized to empty defect (n = 5), (D) Quantification of bone cross-sectional area compared to empty defect (n = 5), (E,F) H&E (inset boxes show where OPN immunostaining in I and J were taken from) and (G, H) Masson’s trichrome staining; Scale bar in E-H = 500 μm, (I, J) Immunofluorescence images of osteopontin staining; Scale bar = 50 μm, (K) Quantification of OPN immunostained area (n = 12), (L) Quantification of OPN signal intensity (n = 12). (*,*** represent p < 0.05, 0.001).