Table 2.
In Vitro Culture and Embryo Transfer of Marmoset Embryos
| References | IVC | Embryo Transfer |
|---|---|---|
| 28 | MEM + 10% human cord serum + insulin-transferrin-sodium selenite at 37°C, 5% CO2 20 CL/21 IVF (95.2%) 3 BL/15 CL (20%, 1 hatched BL) |
75 IU hCG on d 8 ET on d 3 after ovulation date Surgical ET ET at 4-to 6-cell stage (on d 3 IVC) 2 pregnant/3 IVF-ET (66.7%) 2 pregnant/2 in vivo fertilized embryos (100%) |
| 29 | MEM + 10% marmoset serum at 37°C, 5% CO2 60 CL/66 IVF (91%) |
NA |
| 34 | Two-step culture at 37°C, 5% CO2 10% O2 in air (1) First 48 h: TL medium +3 mg/mL BSA + 10% FBS + 0.5 mM sodium pyruvate +1 mM hypotaurine +1 mM glutamine +50 μg/mL gentamycin (2) CMRL-1066 + 20% FBS + 0.5 mM sodium pyruvate +10 mM sodium lactate +1 mM glutamine +1 mM hypotaurine +66 μg/mL penicillin G-K + 50 μg/mL gentamycin 93 CL/97 IVF (95.9%) 5 BL/93 CL (5.4%) |
NA |
| 30 | MEM + 10% female marmoset serum +60 mg/L penicillin +50 mg/L streptomycin at 37°C, 5% CO2 | 75 IU hCG on d 8 ET on d 3 after hCG injection Surgical ET ET at 4-cell stage |
| 35 | Two-step culture at 37°C, 5% CO2 10% O2 in air (1) First 48 h: G1.2 (2) G2.2 medium 47 CL/53 IVF (88.7%) 25 BL/47 CL (53.2%, 18 hatched BL) |
NA |
| 36 | Three-step culture at 37°C, 6% CO2, 5% O2 in air (1) First 72 h: G1.2 medium +5 mg/mL human serum albumin (2) Next 24 h: G2.2 medium +5 mg/mL human serum albumin (3) G2.2 medium +5 mg/mL human serum albumin +10% FBS CL: 44.7% BL: 46.7% |
NA |
| 37 | Two-step culture at 38°C, 5% CO2 in air (1) First 48 h: ISM1 (2) ISM2 + 10% FBS + inactivated marmoset embryonic fibroblast coculture |
NA |
| 38 | ISM medium | Surgical ET 1 pregnant/19 IVF-ET (5.3%) 6 pregnant/61 in vivo fertilized embryos (9.8%) |
| 33 | CMRL-1066 + 26 mM NaHCO4 + 10% FBS + 0.5 mM sodium pyruvate +10 mM sodium lactate +1 mM GlutaMax +1 IU/mL Penicillin +1 μg/mL streptomycin in 5% CO2, 5% O2 in air (1) 19 CL/26 IVF (73.1%) (2) 7 CL/14 IVF (50%) (3) 2 CL/11 IVF (18.2%) (4) 12 CL/29 IVF (41.4%) |
NA |
| 43 | Two-step culture (1) First 48 h: ISM1 (2) ISM2 (1) Waymouth 51 CL/55 IVF (92.7%) 5 BL/51CL (9.8%) (2) POM 43 CL/45 IVF (95.6%) 6 BL/43 CL (14%) |
Surgical ET ET at 8-cell to morula stage 2 pregnant/11 IVF-ET (18.2%) |
| 39 | Two-step culture at 37.5°C, 5% CO2, 5% O2 in air (1) First 48 h: ISM1 (2) ISM2 (1) IVF 69 CL/74 IVF (93.2%) 29 BL/69 CL (42%) (2) ICSI 80 CL/82 ICSI (97.6%) 29 BL/80 CL (36.3%) |
Nonsurgical ET ET at 6-cell to blastocyst stage (1) 6- to 8-cell stage ET: 3 pregnant/8 ICSI-ET (37.5%) (2) Blastocyst stage ET: 4 pregnant/20 ICSI-ET (20%) |
| 42 | CMRL-1066 + 26 mM NaHCO4 + 10% FBS + 0.5 mM sodium pyruvate +10 mM sodium lactate +1 mM GlutaMax +1 mM hypotaurine +1 IU/mL penicillin +1 μg/mL streptomycin in 5% CO2, 5% O2 in air (1) 0 E2: 6 CL/10 IVF (60%) (2) 0.1 E2: 9 CL/11 IVF (82%) (3) 1 E2: 9 CL/12 IVF (75%) (4) 10 E2: 4 CL/7 IVF (57%) |
NA |
| 21 | ISM1 at 37°C, 5% CO2, 5% O2 in air | Nonsurgical ET 19 pregnant/113 IVF-ET (16.8%) |
| 40 | ISM1 at 38°C, 5% CO2, 5% O2 in air 385 CL/551 IVF (80.9%) |
Nonsurgical ET 13 pregnant/51 IVF-ET (25.5%) 9 pregnant/31 in vivo fertilized embryos (29%) |
| 41 | Two-step culture at 38°C, 5% CO2, 5% O2 in air (1) First 48 h: Cleav medium (2) BlastAssist medium +10% FBS + cocultured with inactivated mouse embryonic fibroblasts (1) IVO oocytes 12 CL/14 IVF (85.7%) 0 BL/12 CL (0%) (2) IVM oocytes 16 CL/20 IVF (80%) 4 BL/16 CL (25%) |
NA |
BL = blastocysts; CL = cleaved embryos; ET = embryo transfer; IVC = in vitro culture; MEM = Minimum essential medium; IVO = in vivo matured; NA = not applicable.