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. 2022 Mar 10;10:e12850. doi: 10.7717/peerj.12850

Figure 2. Specificity test of the LAMP assay based on colorimetric changes in calcein dye. The LAMP reaction was performed at 65 °C for 40 min using 300 ng of genomic DNA each.

Figure 2

(A) Specificity test on different serovars of Leptospira. Tube 1: L. interrogans serovar Pomona, 2: L. interrogans serovar Serawak, 3: L. interrogans serovar Canicola, 4: L. interrogans serovar Djasiman, 5: L. interrogans serovar Autumnalis, 6: L. interrogans serovar Australis, 7: L. interrogans serovar Pyrogenes, 8: L. interrogans serovar Lai, 9: L. interrogans serovar Copenhageni, 10: L. interrogans serovar Icterohaemorrhagiae, 11: L. interrogans serovar Bataviae, 12: L. interrogans serovar Hebdomadis, 13: L. kirschneri serovar Grippotyphosa, 14: L. borgpetersenii serovar Hardjo Bovis 15: L. biflexa serovar Patoc, NC: no-template control. (B) Specificity test on non-leptospiral bacteria. Tube 1: L. interrogans Pomona, 2: S. maltophillia ATCC, 3: P. aeruginosa, 4: A. baumannii ATCC, 5: E. coli XL10G, 6: S. pyogenes S28, 7: MRSA, 8: E. faecalis 33420, 9: E. faecium 4867, 10: C. difficile, NC: no-template control. Red square box indicates reaction tubes with calcein color change.