Fig. 2.

Palmitic acid (PA) decreases the ubiquitination of FTO in hepatocytes. A Western blotting analysis of FTO protein expression in HepG2 cells treated with the protein synthesis inhibitor cycloheximide (CHX, 10 µg/mL), and/or the proteasome inhibitor MG132 (10 µM) for 24 h. Band intensities were quantified via densitometry. B HepG2 cells overexpressing FLAG-FTO or negative control vectors were transfected with HA-ubiquitin and treated with PA (0.5 mM) for 12 h. The ubiquitination of FTO was assessed via immunoprecipitation and western blotting analyses