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. 2022 Mar 13;21:29. doi: 10.1186/s12944-022-01640-y

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Knockdown of FTO inhibits lipid accumulation in hepatocytes. A Western blotting analysis of the FTO protein level in HepG2 cells transfected with the FTO shRNA lentiviral (shFTO) or negative control (shNC) vector. B-E Oil Red O staining of HepG2 cells transfected with shFTO or shNC after treatment with palmitic acid (PA) (0.5 mM) or 2% bovine serum albumin (BSA) for 24 h (200x magnification). F Cellular contents of triglycerides (TG) in FTO knockdown and control HepG2 cells stimulated with 2% BSA and PA (0.5 mM) for 24 h (n = 3 independent experiments). *P < 0.05 vs. control group