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. 2022 Mar 12;13(3):231. doi: 10.1038/s41419-022-04675-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A IB analysis of whole-cell lysates (WCLs) derived from Panc-1 cells transfected with the indicated plasmids, which were treated with 10 μM MG132 for 6 h before harvesting. B IB analysis of WCLs derived from Panc-1 cells transfected with Fbxo45 constructs or EV. EV: empty vector. C IB analysis of WCLs derived from Panc-1 cells transfected with Fbxo45 siRNA or the negative control (NC). D qRT-PCR analysis to detect Fbxo45 and USP49 mRNA levels after Fbxo45 depletion in 293 T cells. Data are shown as the mean ± SD of three independent experiments. ***p < 0.001 compared to NC. E IB analysis of WCLs derived from Panc-1 cells after the specified duration of 100 μg/ml cycloheximide (CHX) transfection with Fbxo45 siRNA. F USP49 protein abundance in (E) was quantified and plotted. G IB analysis of immunoprecipitates (IPs) and WCLs derived from Panc-1 cells transfected with the indicated plasmids. Cells were treated with 10 μM MG132 for 6 h before harvesting. H IB analysis of ubiquitination products and WCLs derived from Panc-1 cells transfected with the indicated constructs. Cells were treated with 10 μM MG132 for 6 h before harvesting.