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. 2022 Mar 12;13(3):231. doi: 10.1038/s41419-022-04675-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A IB analysis of WCLs derived from Panc-1 cells transfected with the indicated plasmids. B MTT assays to detect cell proliferation of Panc-1 cells transfected with USP49 siRNAs (left) or USP49 cDNA (right). Data were shown as mean ± SD of three independent experiments. **p < 0.01 compared to control, ***p < 0.001 compared to control. C, D Tunel assays to detect apoptosis of Panc-1 cells transfected with USP49 siRNAs (C) or USP49 cDNA (D). *p < 0.05, **p < 0.01, ***p < 0.001 compared to control. E, F Wound healing assays to analyze cell migration capacity of Panc-1 cells transfected with USP49 siRNAs (E) and USP49 constructs (F). **p < 0.01 compared to control. ***p < 0.001 compared to control. G, H Transwell assays to analyze cell migration and invasion capacity of Panc-1 cells transfected with USP49 siRNAs (G) and USP49 cDNA (H). **p < 0.01 compared to control, ***p < 0.001 compared to control.