Table 2.

Reaction mixture for the KAPA HiFi HotStart ReadyMix PCR Kit

Component	Step I	Step III
2X KAPA HiFi HotStart ReadyMix	12.5 µl	12.5 µl
Template	1 µl (10 ng)	1 µl (Gibson mix)
SDM_Primer_F (10 µM stock)	0.75 µl	0.75 µl
SDM_Primer_R (10 µM stock)	0.75 µl	0.75 µl
ddH2O	Up to 25 µl	Up to 25 µl