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PCR settings for KAPA HiFi HotStart ReadyMix PCR Kit
| Step | Step I | Step III | ||
|---|---|---|---|---|
| Cycles | Temperature/duration | Cycles | Temperature/duration | |
| Initial denaturation | 1 | 95 °C/3 min | 1 | 95 °C/3 min |
| Denaturation | 30 | 98 °C/20 s | 30 | 98 °C/20 s |
| Annealing | 60/56 °C/20 s | 60/56 °C/20 s | ||
| Extension | 72 °C/1 min | 72 °C/2 min | ||
| Final extension | 1 | 72 °C/5 min | 1 | 72 °C/5 min |
