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. 2022 Mar 12;8:112. doi: 10.1038/s41420-022-00906-9

Fig. 1. HDAC11 expression is upregulated and pyroptosis is activated in the aorta of HFD-fed ApoE−/− mice.

Fig. 1

ApoE−/− mice were fed an HFD for 0, 4, 8, or 12 weeks. A Representative images showing the atherosclerotic lesions of aortic root sections with H&E staining and the lipid deposition of aortic root sections with Oil red O staining, ×100. Scale bar indicates 200 μm. B The atherosclerotic lesion area of aortic root sections was analyzed (n = 3). C GSDMD, GSDMD-N, GSDME, and GSDME-N protein expressions in the aorta were determined by Western blotting (n = 3). D The levels of TNF-α, IL-1β, and IL-18 in the serum were determined by ELISA (n = 10). E, F HDAC11 protein and mRNA expressions in the aorta were determined by Western blotting and quantitative real-time PCR (n = 3). G The expression of HDAC11 in aortic intima by immunofluorescent double staining of the aortic sinus of HDAC11 and CD31. The nuclei were stained blue with DAPI. Scale bar indicates 50 μm. *P < 0.05, **P < 0.01 vs. ND group. Results are expressed as mean ± SD.