Skip to main content
. 2022 Mar 12;8:112. doi: 10.1038/s41420-022-00906-9

Fig. 5. HDAC11 knockdown suppresses both NLRP3/caspase-1/GSDMD and caspase-3/GSDME pathways in TNF-α-induced HUVECs.

Fig. 5

HUVECs were treated with TNF-α (20, 40, or 80 ng/mL) for 12 h. A The expressions of NLRP3, ASC, pro-caspase-1, cleaved caspase-1, pro-caspase-3, and cleaved caspase-3 were determined by Western blotting. B NLRP3 mRNA expression was assayed by quantitative real-time PCR. C Caspase-1 and caspase-3 activity were determined using the Caspase-1 activity assay kit and the Caspase-3 activity assay kit. *P < 0.05, **P < 0.01 vs. control group. D HUVECs were stimulated with 40 ng/mL TNF-α for 12 h after their transfection with HDAC11 siRNA or NC siRNA for 48 h. The expressions of NLRP3, ASC, pro-caspase-1, cleaved caspase-1, pro-caspase-3, and cleaved caspase-3 were determined by Western blotting. E NLRP3 mRNA expression was assayed by quantitative real-time PCR. F Caspase-1 and caspase-3 activity were determined using the Caspase-1 activity assay kit and the Caspase-3 activity assay kit. **P < 0.01 vs. NC siRNA group; #P < 0.05, ##P < 0.01 vs. NC siRNA + TNF-α group. Results are expressed as mean ± SD (n = 3).