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. 2022 Mar 12;8:112. doi: 10.1038/s41420-022-00906-9

Fig. 8. ERG knockdown further augmented pyroptosis in TNF-α-induced HUVECs.

Fig. 8

A, B HUVECs were transfected with ERG siRNA or NC siRNA for 48 h, and the protein and mRNA expressions of ERG were determined by western blotting and quantitative real-time PCR. C HUVECs were stimulated with 40 ng/mL TNF-α for 12 h after their transfection with ERG siRNA or NC siRNA. The protein expressions of GSDMD, GSDMD-N, GSDME, and GSDME-N were determined by Western blotting. D The levels of IL-1β, IL-6, and IL-18 in the cellular supernatant were detected by ELISA assay. E The cellular supernatant LDH level was evaluated with a cytotoxicity detection LDH kit. F The representative photographs of double-fluorescent staining with PI (red) and Hoechst 33342 (blue), and the quantification of PI-positive cells, ×200. Scale bar indicates 100 μm. *P < 0.05, **P < 0.01 vs. NC siRNA group; #P < 0.05 vs NC siRNA + TNF-α group. Results are expressed as mean ± SD (n = 3).