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. 2020 Dec 28;26(6):1817–1825. doi: 10.1111/jcmm.16231

FIGURE 4.

FIGURE 4

MAP2K6 was directly targeted by miR‐375, and also positively regulated by circASPH in KGN cells. A, The sequences of miR‐375, WT‐MAP2K6 and MUT‐ MAP2K6. B, The luciferase activity of KGN cells transfected with MAP2K6 WT + miR‐375 mimic, MAP2K6 WT + miR‐375 NC, MAP2K6 MUT + miR‐375 mimic and MAP2K6 MUT + miR‐375 NC. C, The expression levels of MAP2K6 in five PCOS patients and nine non‐PCOS Control group. D, The linear regression between circASPH expression and MAP2K6 expression in five PCOS patients and nine non‐PCOS Control group. E, The MAP2K6 mRNA expression of KGN cells transfected with OE circASPH, OE NC, si circASPH and si NC. F, G, The MAP2K6 protein expression of KGN cells transfected with OE circASPH, OE NC, si circASPH and si NC. All quantitative values were the average of three independent experiments. *P < 0.05 [Colour figure can be viewed at wileyonlinelibrary.com]