FIGURE 2.

The identification of circVRK1. A, qPCR examined circVRK1 expression between PE and NP. B, qPCR examined circVRK1 expression between early‐onset PE, late‐onset PE. C, Sanger sequence examined the circular splicing site of circVRK1. D, qPCR examined circVRK1 expression. VRK1 was digested by RNase R but circVRK1 was not. E, Using convergent or divergent primers, circVRK was amplified in cDNA but not in gDNA by using divergent primers. F, Fluorescence in situ hybridization showed circVRK1 was widely expressed in the cytoplasm of HTR‐8/SVneo cells, the red represents circVRK1. Data are presented as means ± SD. **P < 0.01, ***P < 0.001 [Colour figure can be viewed at wileyonlinelibrary.com]