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. 2021 Mar 18;26(6):1826–1841. doi: 10.1111/jcmm.16454

FIGURE 3.

FIGURE 3

Silencing circVRK1 promoted trophoblast cell migration, invasion and EMT. A and B, qPCR was used to term that the siRNA and the shRNA were specific to circVRK1 but not VRK1. C, Wound assay was used to detect cell migration ability after cells were transfected with circVRK1 knockdown and NC. D, Transwell assays were performed to detect cell migration and invasion abilities in HTR‐8/Svneo cells. E, Western blot assay was used to evaluate the EMT‐related proteins in HTR‐8/Svneo cells. F, Immunofluorescence analysis was used to detect E‐cadherin and vimentin in HTR‐8/Svneo cells. Data are presented as means ± SD. **P < 0.01, ***P < 0.001, ns: no significance [Colour figure can be viewed at wileyonlinelibrary.com]