TABLE 1.

Demographic data of the 23 patients with CNS tumours, including NGS findings of DNA-based and RNA-based mutations.

Age	Gender	Location	Tumour	Grade	PanTrk	LI (%)	DNA-based mutation	RNA-based mutation	TMB	MSI	NTRK	IDH
28	Male	Frontal	Liponeurocytoma	II	Diffuse	90	None	SLCO5A1-NTRK2	low	stable	Detected	Not done
10	Male	Posterior fossa	Pilocytic astrocytoma	I	Diffuse	90	BRAFV600E/TP53	None	medium	none	Not detected	Not done
54	Male	Parietal	Astrocytoma	IV	No	0	IDH1/ATRX/TP53/BCOR/PTCH1	EGFR amplification	medium	stable	Not detected	mutant
33	Male	Frontal	Oligodendroglioma	III	No	0	MET	None	medium	stable	Not detected	mutant
40	Male	Temporal	Astroblastoma	None	No	0	BRAFV600 E	None	low	stable	Not detected	wildtype
8	Male	Posterior fossa	Pilocytic astrocytoma	I	No	0	None	AUTS2-BRAF/PRKAR2B-BRAF	low	stable	Not detected	Not done
52	Male	Frontal	Glioblastoma	IV	Partial	40	TERT/PTEN/FGFR4	AGBL4-NTRK2/BEND5-NTRK2	low	stable	Detected	wildtype
53	Male	Frontal	Glioblastoma	IV	No	0	TP53	None	high	stable	Not detected	wildtype
4	Female	Spinal	Pilocytic astrocytoma	I	No	0	None	None	medium	none	Not detected	Not done
62	Male	Parietal	Glioblastoma	IV	No	0	BRAFV600E/TP53/APC	None	low	stable	Not detected	wildtype
53	Male	Temporal	Pilocytic astrocytoma	I	Focal	10	TERT/	EGFR amplification/CD4-6 gain	low	stable	Not detected	wildtype
45	Female	Parietal	Glioblastoma	IV	Diffuse	85	PTEN	CDK6 gain/EGFR amplification	low	stable	Not detected	wildtype
40	Male	Posterior fossa	Pilocytic astrocytoma	I	Focal	8	BRAFV600 E	None	medium	stable	Not detected	Not done
6	Female	Cerebellar	Medulloblastoma	IV	Diffuse	90	PTCH1	CDK6 gain	low	stable	Not detected	Not done
3	Female	Frontal	Pilocytic astrocytoma	I	Focal	10	None	None	medium	stable	Not detected	Not done
36	Male	Lateral ventricle	Central neurocytoma	II	No	0	None	None	low	stable	Not detected	Not done
45	Male	Temporal	Glioblastoma	IV	No	0	TERT	None	medium	stable	Not detected	wildtype
12	Female	Posterior fossa	Pilocytic astrocytoma	I	No	0	FGFR1	None	low	stable	Not detected	Not done
14	Male	Hypothalamic	Pilocytic astrocytoma	I	No	0	BARD1	KIAA1549-BRAF	low	stable	Not detected	Not done
64	Female	Temporal	Glioblastoma	IV	Focal	10	ATRX/TERT	None	medium	none	Not detected	wildtype
51	Female	Frontal	Astrocytoma	IV	Partial	45	PTEN	None	high	stable	Not detected	mutant
60	Male	Temporal	Astrocytoma	IV	Partial	40	IDH1	None	medium	none	Not detected	mutant
51	Female	Parietal	Astrocytoma	IV	Non	0	IDH1	None	high	stable	Not detected	mutant

LI, labelling index; TMB, tumour-burden; MSI, microsatellite instability.