Fig. 2.

LinEs undergo structural transitions during synchronized azygotic meiosis at 25°C but not at 34°C. (A-C) A representative LinE protein (Rec27) was observed in synchronized azygotic meiotic cells in pat1-114 (A) and pat1-as1 (B) strains at 34°C, or in pat1-as1 strains (C) at 25°C. Cells were collected at the indicated times after meiotic induction and observed with SIM. (A,B) Rec27 (green) show ‘dotty’ nuclear foci at 3.5 h after induction, consistent with ‘dots’ reported previously (Lorenz et al., 2004; Davis et al., 2008; Estreicher et al., 2012; Fowler et al., 2013). Rec27 foci disassemble at ∼5 h (i.e. before MI). Each image is representative of ≥20 cells in each condition. (C) Rec27 ‘dotty’ foci (green) appear at 3 h after induction and increase in number until 6 h and 7 h when they undergo a conformational transition to ‘linear structures’. The linear structures at 7 h are shorter than those in zygotic meiosis at 25°C (Fig. 1A) but are more nearly continuous than those at 34°C (Fig. 1B, Fig. 2A,B) (Lorenz et al., 2004; Davis et al., 2008; Estreicher et al., 2012; Fowler et al., 2013). Each image is representative of ≥50 cells analyzed in each condition. Nuclei are shown by Hoechst 33342 staining (blue). The dotted line represents the cell outline. Scale bars: 2 μm.