Fig. 7.

Recombination-deficient LinE missense mutations reduce LinE linear structures. (A) Rec25-GFP [in wild type (WT) and in rec27 or mug20 missense mutants] and Rec27-GFP (in a rec25 missense mutant) were analyzed in live zygotic cultures at 25°C. At least 20 cells for each strain were analyzed using SIM. None of the mutants form intact LinE linear structures. In rec27-238, rec27-239 and mug20-251, Rec25 shows non-continuous dotty foci. Rec25 in rec27-240 and Rec27 in rec25-236 show a few dot-like foci, but Rec25 and Rec27 still entered the nucleus. In mug20-250, Rec25 forms very few, if any, foci, which are dot-like. The dotted line is the cell outline. See also Fig. S6. Scale bar: 2 μm. (B) Quantification of the length of LinE-GFP in wild type and mutants. Mean of ‘maximum length’ of each cell and ‘mean of long elements’ (≥6 voxels) of each cell are shown. Data are mean±s.e.m. ***P<0.001 (two-tailed unpaired Student's t-test). ‘Maximum length’ and ‘mean of long elements’ are not significantly different among the four LinEs in wild-type cells (P>0.07). See also Table S4.