Figure 3.

Dietary fiber did not ameliorate WNV-induced colonic inflammation despite reducing TNF. Mice were fed on diets either enriched (HF) or deficient (ZF) in dietary fiber for two weeks and then intranasally infected with LD₁₀₀ WNV. WNV viral load (A), colonic gene expression of Ifna (B), Ifng (C), Il6 (D), Tnf (E), Il10 (F) was determined by qPCR (n = 4-5 per group). (G) Colon length of mock-infected (Mock) or 7 dpi LD₁₀₀ WNV-infected (WNV) mice fed on ZF- or HF-diets were assessed (n = 4-5 per group). Scale bar represents 1cm. Data are represented as mean ± SEM with ^∗p < 0.05; ^∗∗p < 0.01; ^$p < 0.001 by two-way ANOVA.