Skip to main content
. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: Exp Hematol. 2021 Feb 8;96:1–12. doi: 10.1016/j.exphem.2021.02.002

Figure 2: Inflammatory stress in the bone marrow leads to increased Sca1 expression on HSCs and progenitors.

Figure 2:

Compared to PBS treated controls, bone marrow cells treated with recombinant IFN show increased levels of Sca1 expression, a stem cell marker commonly used to define hematopoietic stem and progenitor enriched cell populations. Under inflammatory stress, Sca1 is not only further upregulated on HSCs but also expressed on CMPs and GMPs, which typically do not express Sca1 in the steady state. The upregulation of Sca1 on these cells causes a shift of these cells into the LSK gate. Furthermore, IFN treatment modulates the expression of other stem cell markers like CD150 and CD48. Thus, to accurately determine the effects of inflammatory stressors on HSCs and progenitors, it is critical to use further markers to define these populations.