Table 2.
Advantages and disadvantages of isolation and purification methods of polysaccharides.
| Purification methods | Mechanism | Common types | Advantages | Disadvantages | References |
|---|---|---|---|---|---|
| Solvent method | Respective solubility | Organic solvent | Simple operation; cost-effective | Co-precipitation; low reclamation; Solvent waste | (36, 37) |
| Salting-out method | |||||
| Column chromatography | Charger property | Anion exchange column chromatography. | High separation efficiency; simple operation | Small loading amount; slow flow rate | (38, 39) |
| Different molecular sizes and shapes | Gel Column Chromatography | High separation efficiency; widely used | Small loading amount; unsuitable for the separation of mucopolysaccharides | (40, 41) | |
| Molecule affinity property | Affinity chromatography | High separation efficiency; Easily for low-content polysaccharide | Difficult to find suitable ligands | (42, 43) | |
| Membrane technology | Molecular weight distribution | Microfiltration | Simple operation; reaction conditions are mild | Low yield; long time-consuming; membrane fouling | (44, 45) |