FIGURE 2.

Expression of the hypoxia-related proteins in the brain tissue of control and hypoxia-treated animals confirms moderate hypoxic lesion. (A) Representative Western blots of hypoxia-inducible factor-1 alpha (Hif-1α), cytochrome C oxidase subunit-4 isoform-1 (Cox 4-1), and cleaved caspase 3 (Cas 3) from brain samples obtained at 2, 8, and 24 h post hypoxia. The results of quantification of protein signal intensity is shown in correspondence to total protein amount (in Suplementary material suppl. Figure 1). (B) Notable changes in expression of Hif-1α (control 0.078 ± 0.011, hypoxia 0.136 ± 0.008), and (C) Cox 4-1 (control 0.133 ± 0.009, hypoxia 0.051 ± 0.003, p = 0.024, Student’s t-test) were measured in the samples obtained 24 h after the hypoxia. There is no difference between the groups at earlier time points. (D,E) There is no observed difference in Cas 3 expression in the brain tissue between the control and hypoxia group at any time point.