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. 2022 Feb 28;10:810980. doi: 10.3389/fcell.2022.810980

FIGURE 3.

FIGURE 3

The coronal sections of the subventricular zone (SVZ) and microglial cell morphology confirming the affected proliferation pool and limited microglial cell activation after moderate perinatal hypoxia. (A,A’,C,C’) Show a distinct decrease of cell density in the proliferative SVZ in hypoxia-treated animals compared to controls, at 8 h (A,A’) and more pronounced at 24 h (C,C’) after hypoxia. (B,B’) The ionized-calcium-binding-adaptor-molecule-1 (Iba-1) staining 8 h after hypoxia shows less numerous but more ramified microglial cells in SVZ in hypoxia-treated animals. (D,D’) The class D scavenger receptor 68 (CD68) staining 24 h after hypoxia reveals no severe macrophage reactivity in hypoxia-treated animals compared to controls. (E–G’) Display a change in morphology of Iba-1-immunoreactive microglia in the cingulate cortex and SVZ, but not in the corpus callosum. Control animals’ cingulate cortex and SVZ contained intermediate microglia (E,G), while in hypoxia-treated animals, microglia developed a ramified morphology [(E’,G’) arrows], suggesting their precocious maturation. (F,F’) Within the corpus callosum, these cells have the same amoeboid morphology in both groups of animals. The actual image magnification for (A,A’,C,C’) is shown with the scale bar in (C’), presenting 100 µm, for (B,B’,D,D’) is shown in (D) presenting 50 µm, and for (E–G’) scale bar is in (G’) presenting 25 µm.