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. 2022 Feb 28;10:810980. doi: 10.3389/fcell.2022.810980

FIGURE 5.

FIGURE 5

Coronal sections of the mature cingulate cortex at the age of 3.5 months indicate a downregulation of the diffuse Wfa-positive ECM and parvalbumin-positive neuropil, and concomitant upregulation of PNN expression around some neurons and parvalbumin-immunoreactivity of the interneurons’ soma in animals subjected to perinatal hypoxia. (a) Coronal section through cerebrum at the bregma level −1.56 mm display medial cingulate cortex area (MCC). The black rectangle frame indicates the part of the cortex presented below with higher magnification. (b) Schematic presentation of the position of ACC, MCC, and RSC regarding bregma scale and the position from which below presented sections are isolated. (c) Coronal section through cerebrum at the level of bregma −2.76 mm that displays retrosplenial cingulate cortex area (RSC). The black rectangle frame indicates the part of the cortex presented below with the higher magnification. (A–D) Coronal sections stained by cresylic-violet (Nissl modification) showing preserved gross morphology and proper cortical layering in the MCC and RSC in control and hypoxia-treated animals. (A’–D’) Coronal sections histochemically stained with Wisteria floribunda agglutinin (Wfa) show difference in qualitative characteristics of extracellular matrix where Wfa-positive matrix is diffusely present in all cortical layers and in the form of PNN around some neurons of predominantly cortical layers III and V. In contrast, in the hypoxia-treated animals the Wfa-positive extracellular matrix is localized almost exclusively in the form of PNN. The PNN are most frequently around interneurons of layers II/III and even more in layer V, especially prominent in RSC, in hypoxia treated animals compared to controls. (A”–D”) Coronal sections through MCC and RSC stained with parvalbumin-immunohistochemistry (Parv) indicate downregulated diffuse parvalbumin expression in the cortical neuropil of animals subjected to perinatal hypoxia. However, interneurons’ parvalbumin-positive soma were pronounced, especially in cortical layers II/III and V of these animals. Contrary, Parv-positive cingulate neuropil is pronounced in cortical layers II/III and V, but with less pronounced paralbumin expressing interneurons in controls. The actual image magnification from (A–D”), is shown with the scale bar in (D”) presenting 200 µm.