TABLE 1.
List of primary and secondary antibodies used in Western blot and immunohistochemistry.
| Primary antibodies (clone) | Cat. No. | Host, isotype, format | Dilution | Supplier |
|---|---|---|---|---|
| Hif 1 alpha antibody (H1alpha67) | NB100-105 | Mouse monoclonal | 1:500 | Novus biologicals, Centennial, Colorado, US |
| Protein G purified | ||||
| Cox 4-1 | AF5814 | Goat polyclonal unconjugated | 1:200 | R&D systems Minneapolis, Minnesota, US |
| Cleaved caspase-3 (Asp175) | 9661 | Rabbit polyclonal | 1:1,000 | Cell signaling, Leiden, Netherlands |
| Microglia marker anti Iba-1 | 019-19741 | Rabbit | 1:1,000 | FujiFilm wako chemicals, US |
| CD68 | MCA341R | Mouse monoclonal purified | 1:200 | Biorad, Hercules, California US |
| Anti-parvalbumin antibody | ab11427 | Rabbit polyclonal unconjugated | 1:2,000 | Abcam, Cambridge, United Kingdom |
| Biotinylated wisteria floribunda agglutinin (Bio-Wfa) | L1516 | — | diluted 6 µg on 1 ml | Sigma—Aldrich, Saint Louis, US |
| Fluorescein wisteria floribunda lectin (Wfa, Wfl) | FL-1351 | — | diluted 5 µg on 1 ml | Vector laboratories, Inc., Burlingame, US |
| Secondary antibodies | Cat. No. | Host, isotype, format | Dilution | Supplier |
| Peroxidase-affiniPure secondary antibody | 705-035-003 | Donkey anti-goat IgG (H + L) | 1:50,000 | Jackson immunoresearch, Ely, United Kingdom |
| 1:200 | ||||
| Peroxidase-affiniPure secondary antibody | 711-035-152 | Donkey anti-rabbit IgG (H + L) | 1:50,000 | Jackson immunoresearch, Ely, United Kingdom |
| 1:200 | ||||
| Alexa fluor 488, highly cross-adsorbed secondary antibody | A21206 | Donkey anti-rabbit IgG (H + L) | 1:1,000 | Thermo fisher Scientific, Waltham, Massachusetts, US |
| Alexa fluor 546, highly cross-adsorbed secondary antibody | A10040 | Donkey Anti-Rabbit IgG (H + L) | 1:1,000 | Thermo fisher scientific, Waltham, Massachusetts, US |
| Alexa fluor 647, highly cross-adsorbed secondary antibody | A21447 | Donkey Anti-Goat IgG (H + L) | 1:1,000 | Thermo fisher scientific, Waltham, Massachusetts, US |