KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
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| Rabbit anti-DOT1L/KMT4 | Abclonal | Cat#A12329; RRID:AB_2861655 |
| Rabbit anti-β-tubulin | Cell Signaling Technology | Cat#2146; RRID:AB_2210545 |
| Rabbit anti-HA tag | Cell Signaling Technology | Cat#3724; RRID:AB_1549585 |
| Rabbit anti-Histone H3 | Cell Signaling Technology | Cat#9715; RRID:AB_331563 |
| Rabbit anti-H3K79me2 | Millipore | Cat#04–835; RRID:AB_1587126 |
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| Bacterial and virus strains | ||
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| E. coli BL21(DE3)pLysS | Novagen | Cat#69388–3 |
| E. coli B834(DE3)pLysS | Novagen | Cat#69042–3 |
| E. coli HB101 | ATCC | Cat#67593 |
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| Chemicals, peptides, and recombinant proteins | ||
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| Atto647N maleimide | Sigma | Cat#05316 |
| AlexaFluor488 maleimide | Fisher | Cat#A10254 |
| TALON Metal Affinity Resin | Clontech | Cat.#635669 |
| Source S resin | GE Healthcare | Cat.#17094405 |
| Source Q resin | GE Healthcare | Cat.#17094705 |
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| Experimental models: Cell lines | ||
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| MV4;11 | ATCC; gifts from gift from Drs. Xiaobing Shi and Hong Wen; Wan et al. (2017) | Cat#CRL-9591 |
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| Oligonucleotides | ||
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| smFRET nucleosome oligonucleotides (for oligonucleotides used to prepare nucleosomes for smFRET, see Table S1) | This study | N/A |
| RT-qPCR primers (for RT-qPCR primers, see Table S3) | This study | N/A |
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| Recombinant DNA | ||
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| Plasmid expressing DOT1Lcat | Anderson et al. (2019) | pST50Tr-STRaHISNhDot1t1x1 |
| Plasmid expressing DOT1L(1–332) | This study | pST50Tr-STRaHISNhDot1t3 |
| Plasmid expressing DOT1Lcat(R282A) | Anderson et al. (2019) | pST50Tr-STRaHISNhDot1t1x4 |
| Plasmid expressing DOT1Lcat(F326A) | Anderson et al. (2019) | pST50Tr-STRaHISNhDot1t1x6 |
| Plasmid expressing DOT1Lcat(K330A) | Anderson et al. (2019) | pST50Tr-STRaHISNhDot1t1x5 |
| Plasmid expressing DOT1Lcat(C45A, C75A, C179A, N324C) | This study | pST50Tr-STRaHISNhDot1t1x30 |
| Plasmid expressing DOT1Lcat(R282E, C45A, C75A, C179A, N324C) | This study | pST50Tr-STRaHISNhDot1t1x33 |
| Plasmid expressing DOT1Lcat(F326A, C45A, C75A, C179A, N324C) | This study | pST50Tr-STRaHISNhDot1t1x31 |
| Plasmid expressing DOT1L(1–332, C45A, C75A, C179A, N324C) | This study | pST50Tr-STRaHISNhDot1t3x30 |
| Plasmid expressing DOT1Lcat(C45A, C75A, C179A, E69C) | This study | pST50Tr-STRaHISNhDot1t1x26 |
| Plasmid expressing DOT1Lcat(R282E, C45A, C75A, C179A, E69C) | This study | pST50Tr-STRaHISNhDot1t1x37 |
| Plasmid expressing DOT1Lcat(F326A, C45A, C75A, C179A, E69C) | This study | pST50Tr-STRaHISNhDot1t1x39 |
| Plasmid expressing DOT1Lcat(G163R, G165R, C45A, C75A, C179A, E69C) | This study | pST50Tr-STRaHISNhDot1t1x38 |
| Plasmid expressing DOT1L(1–332, C45A, C75A, C179A, E69C) | This study | pST50Tr-STRaHISNhDot1t3x26 |
| Plasmid expressing H2A(L65C) | This study | pST50Trc4-hH2A.Dx11 |
| Plasmid expressing H3(K79M) | This study | pST50Tr-hH3.2x26 |
| Lentiviral plasmid expressing DOT1L-targeted sgRNA | This study | LRG2.1-sgDOT1L |
| Retroviral plasmid expression murine Dot1L | This study | pMSCVneo-KHAmDot1L |
| Retroviral plasmid expression murine Dot1L(R282A) | This study | pMSCVneo-KHAmDot1Lx1 |
| Retroviral plasmid expression murine Dot1L(R282E) | This study | pMSCVneo-KHAmDot1Lx2 |
| Retroviral plasmid expression murine Dot1L(F326A) | This study | pMSCVneo-KHAmDot1Lx4 |
| Retroviral plasmid expression murine Dot1L(I290A) | This study | pMSCVneo-KHAmDot1Lx5 |
| Retroviral plasmid expression murine Dot1L(K330A) | This study | pMSCVneo-KHAmDot1Lx9 |
| Retroviral plasmid expression murine Dot1L(G163R/G165R) | This study | pMSCVneo-KKHAmDot1Lx7 |
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| Software and algorithms | ||
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| Microsoft Excel | Microsoft | https://products.office.com/en-us/excel |
| ImageJ | NIH | https://imagej.nih.gov/ij/ |
| Matlab R2021a | Mathworks | https://www.mathworks.com |