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. Author manuscript; available in PMC: 2022 Mar 14.
Published in final edited form as: Methods Mol Biol. 2020;2063:139–154. doi: 10.1007/978-1-0716-0138-9_11

Figure 2. Western blot analysis of subcellular marker proteins in each organelle fraction.

Figure 2.

Several conventionally recognized organelle marker proteins were used to determine the purity of the various subcellular fractions. A highly purified fraction is characterized by 1) enrichment of the corresponding organelle marker in that fraction and 2) the relative absence of markers associated with other organelles. The Western blots presented here represent a typical distribution of marker proteins in human brain cortical tissue (a) and rat brain cortical tissue (b).