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TaqMan® microRNA assay with preamplification is a highly sensitive method for detection of miRNAs from a relatively small quantity of starting total RNA (e.g. from mitochondria and MAM). The amplification plots presented in this figure demonstrate robust detection of miR-146a and miR-107 using 30 ng of total RNA isolated from purified mitochondria, MAM, ER, and cytosol fractions obtained from a 0.5 g human brain tissue sample.
