Methodology for Subcellular Fractionation and microRNA Examination of Mitochondria, Mitochondria Associated ER Membrane (MAM), ER, and Cytosol from Human Brain

. Author manuscript; available in PMC: 2022 Mar 14.

Published in final edited form as: Methods Mol Biol. 2020;2063:139–154. doi: 10.1007/978-1-0716-0138-9_11

	For 1 reaction:
Nuclease-free water	0.20 μl
10✕ Reverse Transcription Buffer	0.80 μl
RNase Inhibitor, 20 U/μl	0.10 μl
100mM dNTPs (with dTTP)	0.20 μl
MgCl₂ (25 mM)	0.90 μl
Megaplex^™ RT Primers (10✕) or custom RT primer pool	0.80 μl
MultiScribe^™ Reverse Transcriptase, 50 U/μl	1.50 μl