Methodology for Subcellular Fractionation and microRNA Examination of Mitochondria, Mitochondria Associated ER Membrane (MAM), ER, and Cytosol from Human Brain

. Author manuscript; available in PMC: 2022 Mar 14.

Published in final edited form as: Methods Mol Biol. 2020;2063:139–154. doi: 10.1007/978-1-0716-0138-9_11

	For 1 reaction:
Nuclease-free water	2.50 μl
2✕ TaqMan® Universal Master Mix II, (No AmpErase® UNG)	5.00 μl
20✕ TaqMan® MicroRNA Assays	0.50 μl