| Antibodies |
|
| Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in goat |
Sigma-Aldrich |
Cat# A8924, RRID:AB_258426 |
| Alexa Fluor® 488 anti-mouse CD19 Antibody, Isotype Rat IgG2a, κ, clone 6D5 |
BioLegend |
Cat# MCA1439A488T, RRID:AB_1101006 |
| PerCP/cyanine5.5 anti-mouse CD8α Antibody, Isotype Rat IgG2a, κ, clone 53–6.7 |
BioLegend |
Cat# 100734, RRID:AB_2075238 |
| Brilliant Violet 605™ anti-mouse CD4 antibody, Rat IgG2a, κ, clone RM4-5 |
BioLegend |
Cat# 100548, RRID:AB_2563054 |
| APC anti-mouse IFN-gamma antibody, Rat IgG1, κ, clone XMG1.2 |
BioLegend |
Cat# 505810, RRID:AB_315404 |
| PE anti-mouse TNF-alpha antibody, Rat IgG1, κ, clone MP6-XT22 |
BioLegend |
Cat# 506306, RRID:AB_315427 |
| PE mouse anti-human Granzyme B antibody, clone GB11 |
BD Biosciences |
Cat# 561142, RRID:AB_10561690 |
|
| Bacterial and virus strains |
|
| POWV strain LB |
Ebel laboratory |
Mandl et al., 1993
|
| POWV-DTV strain Spooner |
Ebel laboratory |
Ebel et al., 1999
|
| Rift Valley Fever Virus vaccine strain (MP-12) |
A. Hise & M. Buller |
Ermler et al., 2013
|
|
| Chemicals, peptides, and recombinant proteins |
|
| POWV peptide library, accession #: NP_620099.1
|
21st Century Biochemicals |
N/A |
|
| Experimental models: Cell lines |
|
| BHK-21 clone 13 |
ATCC |
ATCC Cat# CCL-10, RRID:CVCL_1915 |
| Vero CCL-81™ |
ATCC |
Cat# CCL-81, RRID:CVCL_0059 |
| RMA-S Cells |
ATCC |
RRID:CVCL_2180 |
|
| Experimental models: Organisms/strains |
|
| C57BL/6J mice |
The Jackson Laboratory |
000664 |
| B6.129S7-Rag1tm1Mom/J mice |
The Jackson Laboratory |
002216 |
| NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ mice |
The Jackson Laboratory |
005557 |
|
| Oligonucleotides |
|
| POWV LB qPCR primer 5’ - GGCTGCAAATGAGACCAATTC −3′ |
IDT |
N/A |
| POWV LB qPCR primer 5′’-CAGCGACACATCTCCATAGTC −3′ |
IDT |
N/A |
| POWV LB qPCR probe 5’-/56-FAM/TGGCATCCG/Zen/AGAAAGTGATCCTGC/3IABkFQ/-3’ |
IDT |
N/A |
| POWV Spooner qPCR primer 5′- GCAGCACCATAGGTAGAATGT-3′ |
IDT |
N/A |
| POWV Spooner qPCR primer 5′-CCACCCACTGAACCAAAGT-3′ |
IDT |
N/A |
| POWV Spooner qPCR probe 5’ -/56-FAM/TCTCAGTGG/Zen/TTGGAGAACACGCAT/3IABkFQ-3′ |
IDT |
N/A |
|
| Other |
|
| POWV-E282 H2-Db Tetramer, Alexa Fluor® 647 |
NIH Tetramer Core |
N/A |
