Figure 1.

Typical curves of oxygen consumption by mouse brain mitochondria with the substrates of complex I (2.5 mM potassium malate + 2.5 mM glutamate) (a) or complex II (5 mM potassium succinate) (b). Final volume: 2 ml, temperature 25 °C, 0.5 mg/ml of mitochondrial protein. The medium contained 100 mM КСl, 75 mM mannitol 25 mM sucrose 0.5 mM EGTA-К, 5 mM KH₂PO₄, and 10 mM Hepes/KOH, pH 7.4. Additions: 0.2 mM ADP and 50 μM 2,4-dinitrophenol (DNP). (A color version of this figure is available in the online journal.)