Table 1.

Rates of respiration and parameters of oxidative phosphorylation of brain mitochondria of NMRI mice in the presence of substrates of complex I (potassium malate + potassium glutamate) and complex II (potassium succinate) of the respiratory chain.

Substrates	Respiration rates, nmol O2/min⋅mg			ADP/O	TPhosp. (s)
	State 3	State 4	State 3UDNP
Malate + glutamate	42.32 ± 2.24	6.86 ± 0.80	39.65 ± 2.10	2.95 ± 0.05	54.32 ± 3.10
succinate	64.57 ± 3.26	15.10 ± 1.92	67.08 ± 8.12	1.92 ± 0.05	58.74 ± 3.13

The incubation medium contained 100 mM KCl, 75 mM mannitol, 25 mM sucrose, 5 mM KH₂PO₄, 0.5 mM EGTA, 10 mM Hepes/KOH (pH 7.4). Additions: 2.5 mM potassium malate + 2.5 mM potassium glutamate or 5 mM potassium succinate + 1 µM rotenone, 200 µM ADP, and 50 µM 2,4-dinitrophenol (DNP). The concentration of mitochondrial protein in the cuvette was 0.5 mg/ml. Mitochondrial respiration rates, nmol O₂/min⋅mg; ADP/O, µM/nmol O; T_phos, s. Data are presented as the means ± S.E.M. (n = 3).