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. Author manuscript; available in PMC: 2022 Mar 14.
Published in final edited form as: Dev Biol. 2018 Jun 7;441(1):52–66. doi: 10.1016/j.ydbio.2018.06.006

Fig. 3.

Fig. 3.

CDK-11.1 is required for normal gametogenesis. Hermaphrodites were synchronized at the L1 stage and grown for 3 days at 20 °C before analysis. (A and B) DIC images of wild-type gonads which invariably display a single row of cuboidal oocytes (o) in the proximal end of the germ line, and embryos (e) in the uterus. (C) An image of a wild-type spermatheca. Contained within are a population of sperm with uniform size. (D and E) DIC images of cdk-11.1(tm5495) hermaphrodite gonads. Although some appear normal (panel D) others display fewer oocytes that are less uniform in size and with a disorganized arrangement in the proximal germ line (panel E). (F) An image of a cdk-11.1(tm5495) spermatheca containing some normal looking sperm and some larger cells (asterisks). (G and H) DIC images of cdk-11.1(tm5495)/(bs107) hermaphrodite gonads. Some cdk-11.1(tm5495)/(bs107) trans-heterozygotes display normal-looking oocytes (panel G) while others possess fewer oocytes whose shape, size, and arrangement vary (panel H). (I) An image of a cdk-11.1(tm5495)/(bs107) spermatheca containing normal-sized sperm and some abnormally large cells (asterisks). (J) Quantitation of oocyte defects in various strains. (K) DAPI-stained spermatheca from a cdk-11.1(tm5495) hermaphrodite. Asterisks mark residual bodies which lack DNA (green). Scale bars are 25 μm (panels A–I) and 5 μm (panel K).