Fig. 5.
The MDC1-regulated SCML2 network shifts from the XY-body to the autosome in late-pachytene mutant spermatocytes. (A) MDC1-mediated regulation of USP7 and H2AK119ub via SCML2. (B) Immunocytochemistry of USP7 and localization pattern quantification by stage in 30 dpp wild-type and Adad2M/M spermatocyte (three samples per genotype; USP7, green; SYCP3, red; DAPI, blue) showing decreased frequency of XY enrichment and increased frequency of autosomal USP7 signal. Circles indicate the region of the X and Y chromosomes, defined by morphological parameters. (C) Immunocytochemistry of H2AK119ub and localization pattern quantification by stage in 30 dpp wild-type and Adad2M/M spermatocyte spreads (three samples per genotype; H2AK119ub, green; SYCP3, red; DAPI, blue) showing reduced frequency of H2AK119Ub XY-body exclusion in mutant late meiotic spermatocytes. Images are representative of localization pattern observed in wild type versus mutant. Dashed circles indicate the region containing the X and Y chromosome. Data are mean±s.d. Dots represent frequencies within individuals. Significance was calculated using an unpaired, two-tailed Student‘s t-test. *P<0.05.