Fig. 6.
Mutant spermatocytes fail to localize H3K4me2 to the XY-body late in meiosis. (A) H3K4me2 immunocytochemistry (H3K4me2, green; SYCP3, red; DAPI, blue; dashed circles indicate regions containing the X and Y chromosome). (B) Quantification of localization pattern in 30 dpp wild-type and Adad2M/M spermatocytes (biological triplicate per genotype) showing almost complete lack of XY-body accumulation in mutant spermatocytes late in meiosis. Images are representative of the localization pattern observed in wild type versus mutant. Data are mean±s.d. Dots represent frequencies within individuals. Significance is calculated using an unpaired, two-tailed Student‘s t-test. ***P<0.0001.