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Non-parenchymal cell (NPC) purification. (A) Density gradient. An 8.2% solution of Optiprep is carefully pipetted over cells resuspended in a 17.6% solution of Optiprep. (B) NPCs are concentrated in the interface between the two concentrations of Optiprep (arrow). Any remaining parenchymal cells are pelleted at the bottom of the tube. (C). Final NPC pellet
