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. 2022 Feb 14;13(3):492–498. doi: 10.1021/acsmedchemlett.1c00705

Figure 2.

Figure 2

Results of a luciferase reporter assay of the activities of myostatin inhibitory d-peptides. (A) 7c-ri, (B) MID-35, and (C) MID-36 and MID-39. Cells, HEK293-cell-transfected Smad-responsive reporter (pGL4.48[luc2P/SBE/Hygro]) and control (pGL4.74[hRluc/TK]) plasmids; peptide concentration, 0.3 μM; positive control (prodomain, mouse recombinant) concentration, 10 nM; myostatin concentration, 8 ng/mL (0.32 nM); starvation with serum-free DMEM, 8 h; incubation with or without peptides, 4 h. Values represent means ± SD (N = 3).