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. 2022 Feb 14;13(3):492–498. doi: 10.1021/acsmedchemlett.1c00705

Figure 4.

Figure 4

(A,B) Representative analytical RP-HPLC chromatograms of (A) MIPE-1686 and (B) MID-35 incubated in a solution of trypsin from bovine pancreas (1 μg/mL) on 50 mM Tris buffer (pH 7.5) containing 150 mM NaCl, 10 mM CaCl2, and 0.05% Brij-35 at 37 °C for 400 min. Column, COSMOSIL 5C18-AR-II (4.6 × 150 mm); binary solvent system, a linear gradient of CH3CN (25–40%, 30 min) in 0.1% aqueous TFA; flow, 1.0 mL/min; detection, UV 220 nm. Asterisks identify unknown peaks. (C) Sequence and mass spectrometric data of metabolite tm1 derived from MIPE-1686.