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. 2022 Mar 14;23:78. doi: 10.1186/s13059-022-02646-6

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Developmental basis of shoot apical meristem maturation in single and higher-order mutants of TFAMs. A, B Stereoscope images of meristems (A) and quantification data of leave primordium production for flower transition (B) from WT and tfam single, double, and triple mutants. Data are means ± SD (n = 69, 46, 46, 42, 59, 54, 39, 73, *P < 0.05, **P < 0.01, ***P < 0.001, Student t-test). Scale bars, 100 μm. L, leaf. C Young inflorescences (upper) and diagrams (bottom) of WT and tfam mutants. Colored dots indicate terminated FM (red, orange and yellow dots) and initiated sympodial inflorescence meristem (SIM) (blue and green dots). White dots indicate the first sympodial shoot meristem (SYM). The black arrow indicates continued SIM reiteration. Scale bars, 100 μm. D, E Stereoscope images of floral meristem (D) and quantification of leaf production for flower transition (E) from tmf and tfam mutants. Data are means ± SD (n = 53, 42, 29, 29, 62, **P < 0.01, ***P < 0.001, Student t-test). Red arrowheads indicating the leaf sepal at floral meristem stage. Scale bars, 100 μm. L, leaf