Obesogenic phenotype in female mice ancestrally exposed to TBT. Weekly body weight and body composition in animals ancestrally exposed to 5 nM TBT (A, C) and 50 nM TBT (B, D) compared to control group (DMSO). (E) Percentage of body fat loss after ~16 h of fasting at 28 weeks of age. (F) Plasma levels of leptin at 28 weeks of age. Statistical significance was determined by two-way ANOVA followed by Dunnett’s post-test in panels A–D. Statistical significance was determined by Kruskal-Wallis test followed by Dunn’s post-test in panels E–F. Data are presented as mean ± s.e.m. *p < 0.05, **p < 0.01. BW: Body Weight; SD: Standard diet; HFD: Higher fat diet. Panels A–E: Control n = 14, TBT5 n = 10, TBT50 n = 10; Panel F: Control n = 14, TBT5 n = 9, TBT50 n = 10.