Two-dimensional PLS-DA scores plot of T2 study plasma samples integrated 1H NMR spectra at 8 (top) and 33 (bottom) weeks of age. Left column: Male samples; Right column: Female samples. Each dot represents an observation (animal), projected onto first (horizontal axis) and second (vertical axis) PLS-DA components. Groups are shown in different symbols and colors. The black ellipse determines the 95% confidence interval, which is drawn using Hotelling’s T2 statistic. A. 8-weeks male samples (Control: n = 14, TBT50: n = 15, 2 latent components, R2Y = 95.3%, Q2 = 0.80). B. 8-weeks female samples (Control: n = 12, TBT50: n = 14, 2 latent components, R2Y = 99.1%, Q2 = 0.98). C. 33-weeks male samples (Control: n = 10, TBT50: n = 12, no robust model). D. 33-weeks female samples (Control: n = 5, TBT50: n = 12, 2 latent components, R2Y = 99.8%, Q2 = 0.99).