Figure 6.

Different impacts of PERK blockade in the two phases of MDSC generation. (A and B) A two-step workflow to induce naive LSK cells to become suppressive MDSCs (A). Naive LSK cells received different combinations of first- and second-phase stimuli. The suppressive capability of the generated myeloid cells was tested at the end of the culture period (B). (C–E) Naive LSK cells were induced with SSN and TES from Hepa mice to become suppressive MDSCs (C). In this process, cells were treated with PERK inhibitors (D) or transfected with siRNAs against PERK (E) in the first and/or second phase of stimulation. The suppressive capability of the generated myeloid cells was tested at the end of the culture. Veh, vehicle; Null, no transfection. Error bars indicate means ± SEM. Data are from four independent experiments (n = 4 samples per group, with cells pooled from three to five mice).