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. 2022 Mar 10;219(4):e20211498. doi: 10.1084/jem.20211498

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© 2022 Liu et al

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Figure S3. — Robust PERK activation promotes myelopoiesis in splenic LSK cells. (A) Effects of 4μ8C and GSK on splenic LSK cell survival, determined by flow cytometry (n = 4 mice per group). Veh, vehicle. (B) Flow cytometry plots, related to Fig. 3 A. Numbers in the plots indicate the proportions of gated cells. (C and D) Proportions of subsets in splenic LSK cells (C): percentage of GM-CSF–expressing cells in splenic MPP3 population (D) from LLC mice. (E) Suppressive activity of PMN-like descendants of splenic LSK cells from LLC mice (n = 3 samples, with cells pooled from three mice). Error bars indicate the mean ± SEM. Statistics: One-way (A) or two-way (C–E) ANOVA corrected by Tukey’s test. **, P < 0.01; ***, P < 0.001. Data are from two independent experiments (A, C, and D), or three independent experiments (E) or are representative of two independent experiments (B). LT, long term; SP, spleen; ST, short term; T, tumor.