Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Mar 14;20:124. doi: 10.1186/s12967-022-03326-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 1 — Generation and functional validation of dual-CAR NK-92. A Structural composition of the BCMA- and CD19-specific CARs used for the dual-CAR approach. B High CAR expression in transfected NK-92 cells, 24 h after electroporation with 50 µg/mL CAR-encoding mRNA (N = 26–28). C CD19 and BCMA-CAR surface expression kinetics in NK-92 over four days (N = 1). D Only target cells expressing cognate antigen are lysed, confirming CAR specificity. Statistical analysis was performed using ANOVA with Tukey’s correction for multiple comparisons (N = 3). E CAR expression of NK-92 transfected with mRNA encoding one (CD19-CAR NK-92 and BCMA-CAR NK-92) or both CARs (dual-CAR NK-92). Expression for dual-CAR NK-92 represents cells positive for both CARs (N = 19). F Dual-CAR NK-92 lyse BCMA⁺CD19⁺ (Daudi), as well as single BCMA⁺ (BCMA-K562) or CD19⁺ (CD19-K562) tumor cells (N = 3). Statistical analysis was performed using ANOVA with Dunnett’s correction for multiple comparisons relative to the dual-CAR NK-92 condition. *p < 0.05. **p < 0.01. ****p < 0.0001. BCMA: B-cell maturation antigen; CAR: chimeric antigen receptor; CS: co-stimulatory domain; H: hinge domain; scFv: single-chain variable fragment; SD: signaling domain; SP: signal peptide; TM: transmembrane domain